Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Apr 15;21(8):2726. doi: 10.3390/ijms21082726

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Trafficking of sucrase-isomaltase (SI) and dipeptidyl peptidase-4 (DPPIV) is impaired upon dextran sulfate sodium (DSS) treatment of Caco-2 cells. (A) Biosynthetic labeling of SI. Caco-2 cells that were treated or not treated with DSS were labeled for 6 or 8 h with 35S-methionine, and the detergent extracts were immunoprecipitated with anti-SI antibodies. The immunoprecipitates were treated or not treated with Endo H and finally subjected to SDS-PAGE and fluorography. (B) Biosynthetic labeling of DPPIV. Caco-2 cells that were treated or not treated with DSS were labeled for 15, 30, 60, 90, and 120 min with 35S-methionine and the cellular extracts immunoprecipitated with anti-DPPIV antibody. Here again, the immunoprecipitated proteins were treated with Endo H and subjected to SDS-PAGE and fluorography.