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. 2020 Apr 15;21(8):2726. doi: 10.3390/ijms21082726

Figure 6.

Figure 6

Lipid rafts are altered in dextran sulfate sodium (DSS)-treated cells. (A) Control or DSS-treated Caco-2 cells were solubilized with 1% (w/v) Triton X-100 and run on sucrose density gradients. Ten fractions were collected and analyzed for distribution of the lipid rafts marker FLOT2 by immunoblotting (IB). FLOT2 is redistributed from fractions 1–3 in control to fractions 2 and 3 (and slightly also 4). The redistribution suggests an altered lipid rafts composition in DSS-treated cells. (B) Total lipids were isolated from sucrose gradient fractions obtained from Triton X-100 lysates as described in A). The proportion of cholesterol in the first three fractions (lipid rafts or detergent-resistant membranes/DRM) and in the last three fractions (detergent soluble membranes/DSM) in the WT cells was compared with that of cholesterol from the same fractions in cells treated with DSS. Cholesterol analysis was performed by HPLC (Student’s t-test, *** p < 0.05, SEM, n = 3). A significant reduction (50%) in the cholesterol levels was observed in the DRM of DSS-treated Caco-2 cells.