Figure 2.

miR-26a and Let-7a inhibit cell proliferation and motility by targeting HMGA1. (A) qRT-PCR (upper panel) and immunoblot (lower panel) for HMGA1 after scrambled, mir-26a and Let-7a transfections. mRNA and proteins were extracted from the scrambled-, miR-26a-, Let-7a- transfected TCam-2 cells 48 h after transfection. For immunoblots the level of α-tubulin was used as loading control. The results are reported as the mean of expression values with error bars indicating SD; n = 4. * p < 0.05. (B) TCam-2 cells proliferation was assayed in miR-26a- and Let-7a-transfected cells, compared with scrambled-transfected cells. The cell proliferation results are reported as the mean of expression values with error bars indicating SD; n = 6. ** p < 0.01. (C) Migration and invasion capabilities were assayed in scrambled-, miR-26a- and Let-7a-transfected cells. Scale bar: 100 μm. A representative experiment is showed.