Figure 6.

Evaluation of the concentration of β-lactam antibiotics incubated with 5 μg/mL of intact OMVs from RC85⁺ and isogenic mutant strains of RC85⁺ (Δblc1, ΔompC, and ΔompF). The initial concentrations and certain time points for measurement were as follows: ampicillin, 30 μg/mL, 5 h (a); cefotaxime, 1.25 μg/mL, 4 h (b); cefoperazone, 4 μg/mL, 3 h (c); amoxicillin, 12 μg/mL, 5 h (d); cefazolin, 8 μg/mL, 1 h (e); cefalexin, 16 μg/mL, 11 h (f). Respective antibiotics without OMVs were averaged and normalized as 100%, and the corresponding concentration of antibiotics with OMVs were calculated. The data are presented as means and SEMs of three independent experiments. The abbreviation ‘ns’ means not significant. ** p < 0.01, *** p < 0.001 and **** p < 0.0001.