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. 2020 Apr 23;21(8):2983. doi: 10.3390/ijms21082983

Figure 1.

Figure 1

Carbonic anhydrase IX (CA-IX) and CA-I basal expression in MDA-MB-231 and A549 cells. (A). Protein expression of CA-IX and CA-I in MDA-MB-231 and A549 cells cultured for 24 h in complete medium with 1% FBS. (B). Quantification of CA-IX and CA-I protein expression. β-actin was used to normalize loading. Arbitrary Densitometry Units (A.D.U.) ± SD were reported as protein of interest vs β-actin. (n = 3). (C,D). HIF-1α and CA-IX protein expression in normoxic and hypoxic conditions in MDA-MB-231 (C) and A549 (D) cells. Tumor cells were treated with increasing doses of CoCl2 [100–200 µM], under experimental condition of DMEM with 1% FBS, for 24 and 48h. Numbers represent protein quantification reported as Arbitrary Densitometry Units (A.D.U.) ± SD of the protein of interest/β-actin vs the basal control condition (Ctr). (n = 3). * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. untreated cells (Ctr).