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. 2020 Apr 23;21(8):2983. doi: 10.3390/ijms21082983

Figure 7.

Figure 7

CA-IX pharmacological inhibitors control the mesenchymal phenotype of MDA-MB-231 and A549 cells. (A,B). Basal expression of E-Cadherin inMDA-MB-231 and A549 cells, cultured in medium with 1% FBS (n = 3). Arbitrary Densitometry Units (A.D.U.) ± SD were reported as protein of interest/β-actin. *** p < 0.001 A549 vs MDA-MB-231. (C,D). MDA-MB-231 and (E,F) A549 were treated with SLC-0111 and AA-06-05 [100–200 µM] for 24 h to evaluate the expression of cell-adhesion pathway: Fibronectin, E-Cadherin, Vimentin. The experiments were performed under condition of medium with 1% FBS. Signals were evaluated through western blot and β-actin was used to normalize protein loading. For each experimental condition Arbitrary Densitometry Units (A.D.U.) ± SD were reported as protein of interest/ β-actin vs. basal control. (n = 3). * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. untreated cells (Ctr).