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. 2020 Apr 23;21(8):2983. doi: 10.3390/ijms21082983

Figure 8.

Figure 8

Zymographic analysis of conditioned medium from cultured MDA-MB-231 and A549 cells treated with CA-IX pharmacological inhibitors. Tumor cells were treated with increasing concentrations of SLC-0111 [50–200 µM] and AA-06-05 [25–200 µM], in serum-free medium. Conditioned medium was collected, in triplicate, after 48h and analyzed by gelatin zymography. MMP-2 activity in conditioned media of MDA-MB-231 (A,B) and A549 (D,E) was assessed as lysis bands on blue background and quantified in the inverted images as optical density (± SD)/total proteins in the sample. (n = 3). * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. untreated cells (Ctr). Western blot analysis was performed in parallel experiments to evaluate the release of TIMP-2 in the conditioned medium of MDA-MB-231 (C) and A549 (F). For each experimental condition TIMP-2 release was reported as optical density (± SD)/total proteins in the sample vs. basal control. (n = 3). * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. untreated cells (Ctr).