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. 2020 Apr 18;21(8):2825. doi: 10.3390/ijms21082825

Figure 4.

Figure 4

Effects of the combination therapy with an AKT inhibitor (AZD5363) and mTOR inhibitors (AZD2014 and BEZ235) on the apoptosis of PI3KCA and mTOR-mutated bladder cancer cells. (A) The combination therapy increased the apoptosis of J82 cells. Apoptosis was detected using Annexin V/propidium iodide staining. Cells were exposed to AZD5363 (3 μM), mTOR inhibitors (1 μM AZD2014 and 0.5 μM BEZ235), or a combination of both for 48 h. (B) Pro-apoptotic protein expression was detected by Western blotting. J82 cells were treated with AZD5363 and mTOR inhibitors for 48 h. Proteins were extracted and Western blotting was performed for cleaved PARP, cleaved caspase-3, Bcl-2, and Bax. β-Actin was used as the loading control.