Table 2.

Maximum capacity of a single qPCR instrument, assuming it is run for 24 h a day. A. A typical diagnostic instrument such as the Roche Lightcycler. B. An extremely fast research qPCR instrument with 48 wells (PCRMax Eco). This instrument stands out as it is one of the fastest qPCR systems available and typical research qPCR systems do not perform like this. More typical research qPCR systems include the Biorad CFX systems, Applied Biosystems StepOnePlus/QuantStudios, etc. All of these are capable of running standard and fast protocols but do not have the higher ramp rates of the Eco to perform such very rapid PCR.

PCR Instrument	Reactions/Run	Daily Throughput	Daily Throughput
e.g., Roche Lightcycler		1.5 h/run	20 min/run
Low throughput	96	1536	6912
Medium throughput	384	6144	27,648
High throughput	1536	24,576	110,592
Rapid 48 Well Instrument	Reactions/Run	Daily Throughput	Daily Throughput
e.g., PCRMax Eco		45 min/run	20 min/run
Throughput	48	1536	3456

Grey colour: a contrast.