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Relative gene expression normalized to GAPDH and day 0 (2−∆∆Ct) of human articular chondrocytes after 7 days of culture in passage 2, 3, and 4 on hydrogel, on polystyrene or in pellet culture. (A) COL2A1 expression in OA chondrocytes; (B) COL2A1 expression in non-OA chondrocytes; (C) COL1A1 expression in OA chondrocytes; (D) COL1A1 expression in non-OA chondrocytes; (E) COL2A1/COL1A1-ratio in OA chondrocytes; (F) COL2A1/COL1A1-ratio in non-OA chondrocytes.
