Table 3.
Deiminated proteins in plasma-EVs of sham-treated rats (Rattus norvegucus), as identified by Figure 95. and LC-MS/MS analysis. Deiminated proteins in sham-treated rat plasma were isolated by immunoprecipitation using the pan-deimination F95 antibody. The resulting F95-enriched eluate was then analysed by LC-MS/MS and peak list files submitted to mascot. Rattus norvegicus species-specific peptide sequence hits are listed, showing number of sequences for protein hits and total score. Green highlighted rows indicate protein hits identified in sham-treated (control) rat plasma only.
| Protein Name | Symbol | Sequences | Total Score (p < 0.05) ⱡ |
|---|---|---|---|
| Serum albumin | A0A0G2JSH5_RAT | 13 | 829 |
| RCG36700 | A0A0G2K3G0_RAT | 13 | 654 |
| Keratin, type II cytoskeletal 5 | K2C5_RAT | 14 | 624 |
| Keratin, type I cytoskeletal 17 | A0A0G2K9Q9_RAT | 12 | 571 |
| Keratin, type I cytoskeletal 14 | K1C14_RAT | 8 | 500 |
| Actin, cytoplasmic 1 | A0A0G2K3K2_RAT | 8 | 461 |
| Keratin, type II cytoskeletal 6A | K2C6A_RAT | 8 | 401 |
| Keratin, type I cytoskeletal 19 | K1C19_RAT | 9 | 391 |
| Keratin 16 | Q6IFU9_RAT | 6 | 368 |
| Heat shock protein HSP 90-beta | A0A0G2K793_RAT | 9 | 337 |
| Keratin, type II cytoskeletal 8 | K2C8_RAT | 6 | 330 |
| Keratin, type II cytoskeletal 75 | A0A0H2UHH5_RAT | 7 | 320 |
| Heat shock 70 kDa protein 1A | HS71A_RAT | 5 | 312 |
| Heat shock protein HSP 90-alpha | HS90A_RAT | 7 | 298 |
| Uncharacterized protein | A0A0G2K828_RAT | 4 | 284 |
| Ig gamma-2A chain C region | IGG2A_RAT | 5 | 281 |
| Isoform LMW of Kininogen-1 | KNG1_RAT | 6 | 272 |
| Alpha-1-macroglobulin | A1M_RAT | 8 | 267 |
| Keratin, type I cytoskeletal 42 | K1C42_RAT | 5 | 265 |
| Heat shock 70 kDa protein 1-like | HS71L_RAT | 4 | 257 |
| Complement C3 | M0RBF1_RAT | 6 | 238 |
| Ig gamma-2C chain C region | IGG2C_RAT | 5 | 224 |
| Serotransferrin | A0A0G2QC06_RAT | 5 | 222 |
| Uncharacterized protein | D4ACR1_RAT | 3 | 179 |
| Murinoglobulin-1 | A0A0G2JUP5_RAT | 3 | 148 |
| Ig kappa chain C region, A allele | KACA_RAT | 2 | 142 |
| Desmoplakin | F1LMV6_RAT | 3 | 131 |
| C-reactive protein | A0A0G2K8V5_RAT | 2 | 119 |
| Histone H4 | H4_RAT | 2 | 109 |
| 14-3-3 protein theta | 1433T_RAT | 2 | 103 |
| 40S ribosomal protein | RSSA_RAT | 1 | 93 |
| Elongation factor 1-alpha | M0R757_RAT | 2 | 87 |
| Ubiquitin-like modifier-activating enzyme 1 | UBA1_RAT | 2 | 87 |
| Ig gamma-2B chain C region | IGG2B_RAT | 2 | 82 |
| Uncharacterized protein | A0A0G2JUY4_RAT | 1 | 82 |
| L-lactate dehydrogenase A chain | LDHA_RAT | 2 | 69 |
| Uncharacterized protein | M0RA79_RAT | 1 | 66 |
| Apolipoprotein E | A0A0G2K151_RAT | 1 | 64 |
| Globin a4 | A0A0G2JSW3_RAT | 1 | 63 |
| Uncharacterized protein | A0A0G2K980_RAT | 1 | 60 |
| Uncharacterized protein | D3ZE63_RAT | 1 | 57 |
| Histone H2A.Z | H2AZ_RAT | 2 | 55 |
| Peptidyl-prolyl cis-trans isomerase FKBP4 | FKBP4_RAT | 1 | 55 |
| Peroxiredoxin-2 | A0A0G2JSH9_RAT | 1 | 55 |
| L-lactate dehydrogenase B chain | LDHB_RAT | 2 | 54 |
| Eukaryotic initiation factor 4A-II | A0A0G2K8B7_RAT | 2 | 52 |
| Clathrin heavy chain | F1M779_RAT | 1 | 49 |
| Tubulin alpha chain | A0A0H2UHM7_RAT | 1 | 49 |
| Uncharacterized protein | A0A096P6M7_RAT | 1 | 46 |
| cAMP responsive element binding protein 3, isoform CRA | A0A0G2K331_RAT | 2 | 45 |
| Transportin 1 | F1LQP9_RAT | 1 | 44 |
| Unconventional myosin-Va | A0A0G2K4Y7_RAT | 2 | 44 |
| 6-phosphogluconate dehydrogenase, decarboxylating | A0A0G2K7Q8_RAT | 1 | 43 |
| Uncharacterized protein | A0A0G2K8S2_RAT | 1 | 41 |
| Phospholipase A2 | A0A0G2JZ44_RAT | 1 | 41 |
| Histone H2B | A0A0G2JXE0_RAT | 1 | 40 |
| Ventricular zone-expressed PH domain-containing protein homolog 1 | A0A0G2K6S2_RAT | 1 | 40 |
| Uncharacterized protein | A0A0G2JVP4_RAT | 1 | 37 |
| Pyruvate kinase | A0A0G2JVG3_RAT | 1 | 37 |
| Centromere protein E | D3ZV60_RAT | 1 | 36 |
| Methyltransferase-like 3 | Q4V8G6_RAT | 1 | 35 |
| Transketolase | G3V826_RAT | 1 | 35 |
| Uncharacterized protein | A0A0G2KB28_RAT | 1 | 35 |
| Enhancer of mRNA-decapping protein 4 | EDC4_RAT | 1 | 35 |
| T-complex protein 1 subunit eta | D4AC23_RAT | 1 | 34 |
| Signal-induced proliferation-associated 1 | E9PSX8_RAT | 1 | 34 |
| Stress-70 protein, mitochondrial | F1M953_RAT | 1 | 32 |
| Filamin-C | A0A0H2UHR7_RAT | 1 | 32 |
ⱡ Ions score is −10*Log(P), where P is the probability that the observed match is a random event. Individual ions scores > 31 indicated identity or extensive homology (p < 0.05). Protein scores were derived from ions scores as a non-probabilistic basis for ranking protein hits.