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. 2020 Apr 22;13(8):1966. doi: 10.3390/ma13081966

Figure 4.

Figure 4

Optical microscope images of 3D-bioprinted osteoblast constructs after cryopreservation: (a) representative MG-63 construct, (b) representative Saos-2 construct and (c) positive Alizarin red S staining of a Saos-2 construct, showing in red colour the salts of calcium minerals. The samples were cryopreserved at day 3 of culture and the images were obtained after 7 days of culture.