Figure 2.

Determination of NOx in the sera from healthy individuals, T1D patients with normal or high glycaemia and effects of these sera on HUVEC permeability. The sera of patients were grouped according to fasting glycaemia. (A) The levels of NOx were measured in the sera from healthy subjects (CTR) and T1D individuals with normal (T1D n.g.) or high glycaemia (T1D h.g.) as described in the methods. (B) Endothelial permeability was measured in HUVEC exposed to 10% of the sera using a Transwell Permeability Assay. The results are the mean of three experiments in triplicate. * p < 0.05.