Figure 3.

NOx release and permeability in HUVEC exposed to different concentrations of glucose. HUVEC were cultured in a medium containing 5 mM (CTR), 11.1 and 30 mM glucose for 24 h. LPS and Bradykinin were used as positive controls. (A) Media were collected and NOx levels were measured as described in the methods. (B) Endothelial permeability was studied as described in the methods. The results are the mean of three experiments in triplicates ± standard deviation (SD). * p < 0.05; ** p < 0.01; *** p < 0.001.