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Permeability in HUVEC cultured in medium containing 10% hyperglycaemic T1D or CTR sera after genetic and pharmacological inhibition of iNOS or eNOS. HUVEC were cultured in the presence of sera from healthy and T1D subjects for 24 h with or without L-NAME or L-NIL (A) or after gene silencing (B). A scrambled non-silencing sequence was used as a control (–) for silencing. The results are the mean of three experiments in triplicate. ** p < 0.01; *** p < 0.001.
