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. 2020 Apr 20;21(8):2864. doi: 10.3390/ijms21082864

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The toxic effects of SAS/LAS. The mortality of embryos treated with 0, 0.05, 0.1, 0.5, 0.75, 1, 10, or 100 µg/mL (A) SAS or (B) LAS at 24, 48, 72, 96, 120 hpf. (C) LC₅₀ of SAS/LAS-treated embryos. The lower LC₅₀ of LAS indicates that the toxicity of LAS is higher than that of SAS at 24, 48, 72, 96, and 120 hpf. The LC₅₀ of SAS/LAS was calculated by IBM SPSS 22 statistics. The mortality was performed from 3 replicate trials using 30 embryos at each dose. Error bars present the SEM of the mean.