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. 2020 Apr 21;21(8):2900. doi: 10.3390/ijms21082900

Figure 1.

Figure 1

Cell survival and expression of brain growth factors in the nerve growth factor-administered PC12 cells. The cells had treatments with 70% ethanol Tetragonia tetragonioides or its water extract with 10 and 50 μg/mL dosages, and after 1 h, cell death was induced by 10 μM amyloid-β(25–35). After 24 h treatment, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and mRNA expression were measured. Amyloid-β(25–35) was administered without Tetragonia tetragonioides Kuntze (TTK) extract (AD-Con), ethanol extract (TTK-E), or water extract (TTK-W), and 10 μM amyloid-β(35–25) was administered without TTK extract (Normal-Con). (A) Cell survival determined by MTT assay. (B) Brain-derived neurotrophic factor (BDNF) mRNA expression. (C) Ciliary neurotrophic factor (CNTF) mRNA expression. (D) Tau mRNA expression. The units for mRNA expression were fold-changes of mRNA expression from the control. Each value represents the mean ± SD (n = 3). # Significantly different among the groups in one-way ANOVA at p < 0.05, ## p < 0.01, and ### p < 0.001. * Significantly different from AD-Con at p < 0.05, ** p < 0.01, and *** p < 0.001 in the Tukey test. † Significantly different from the Normal-Con at p < 0.05 and †† at p < 0.01 in the Tukey test.