Figure 1.

Differences among breast cancer cell-derived matrices. MCF-7 or MDA-MB-231 cells were grown in standard conditions for 72 h, and the respective derived extracellular matrices (ECMs) were obtained, as described in the Methods section. The contents of tenascin-C (TN-C), laminin (LN), fibronectin (FN), osteopontin (OPN), and vitronectin (VT) were assayed by indirect ELISA. The matrix protein content of MDA-ECM is shown as the fold increase relative to MCF-ECM. Numbers at the top of the plots represent an optical density ratio ranging from MCF-ECM:MDA-ECM. The plot is the mean of 3 independent experiments (* p < 0.05).