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. 2020 Apr 23;21(8):2995. doi: 10.3390/ijms21082995

Figure 2.

Figure 2

MDA-MB-231-derived ECM promoted a slight decrease in E-cadherin expression in MCF-7 cells in 48 h. MCF-7 and MDA-MB-231 cells were cultured in standard conditions for 48 h, and decellularized ECMs were obtained, as described in Methods. MCF-7 cells were cultured onto MDA-ECM or onto their own matrix with TGF-β1 (10 ng/mL) for 48 h. (A) Cell morphology was analyzed, and representative images were obtained at 40× magnification. A black asterisk indicates lost or remaining intercellular connections. Scale bar: 20 µm (B–E) Lysates of MCF-7 cultured as described for 48 h were immunoblotted with anti-N-cadherin (B), anti-α-SMA (C), anti-fibronectin (D), and anti-E-cadherin (E) antibodies. The results are shown as the mean fold increase relative to the control (MCF-ECM), and bars represent the mean ± SD calculated from 3 individual experiments (* p < 0.05 and ** p < 0.01).