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. 2020 Apr 23;21(8):2995. doi: 10.3390/ijms21082995

Figure 4.

Figure 4

MDA-MB-231-derived ECM decreased E-cadherin expression in MCF-7 cells independent of TGF-beta receptor activation. MCF-7 and MDA-MB-231 cells were cultured in standard conditions for 72 h, and decellularized ECMs were obtained, as described in the Methods. (A) MCF-7 were seeded on their own matrix for 72 h with or without conditioned medium from MDA-MB-231 cultures. Cell lysates were immunoblotted with anti-E-cadherin and anti-tubulin antibodies. (B) MCF-7 cells were seeded on decellularized MCF-ECM with or without TGF-β1 or MDA-ECM in the presence or absence of the TGF-β receptor inhibitor SB431542 (15 µg/mL) for 72 h. Cell lysates were immunoblotted with anti-E-cadherin and anti-actin antibodies. The results are shown as the mean fold increase relative to the MCF-ECM (* p < 0.05); MCF-ECM treated with SB431542 (# p < 0.05) and MDA-ECM without inhibitor ($ p < 0.05) were calculated from 3 individual experiments.