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. 2020 Apr 23;21(8):2995. doi: 10.3390/ijms21082995

Figure 8.

Figure 8

Involvement of integrin αvβ3 in EMT of MCF-7 cells cultured on MDA-ECM. MCF-ECM and MDA-ECM were obtained, as described in the Methods section. MCF-7 cells were cultured on their own ECM with or without TGF-β1 or on MDA-ECM and treated with Kistrin for 72 h (0.4 µM). Cell lysates were immunoblotted with (A) anti-E-cadherin and (C) anti-p-SMAD2 antibodies. The results are shown as the mean fold increase relative to controls: MCF-ECM (* p < 0.05), MCF-ECM + Kistrin (# p < 0.05),and MDA-ECM ($ p < 0.05), calculated from 3 individual experiments. (B) Representative images of MCF-7 cell cultures of each experimental group, treated or not with Kistrin (0.4 µM), at 10× magnification. A black asterisk indicates lost or remaining intercellular connections. Scale bar: 20 µm.