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Combined analysis of messenger RNA(mRNA) and microRNAs (miRNAs). Firstly, we set up miRNAs and mRNA sequencing analysis of several groups with the same comparative combination to conduct miRNA–mRNA association analysis. Then, if the transcriptome analysis of the group comparison results in differential genes, and the miRNAs analysis results in differential miRNAs and corresponding target genes, the integration analysis of miRNAs and mRNA could be performed for the group comparison.
