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. 2020 Apr 20;21(8):2873. doi: 10.3390/ijms21082873

Figure 2.

Figure 2

A typical workflow for protein abundance quantification using Trans-Proteomic Pipeline (TPP). After the raw data files are converted to one of the supported open XML formats, the data are processed with a designated search engine and the search results are validated at spectrum, peptide, and protein level. Meanwhile, the quantification tools will perform the protein quantification with the normalized data. The final output are ready for various downstream analysis.