Figure 5.

Immunogold electron microscopy of cell supernatant containing EVs derived from HUVEC treated with USMB (0.7 MPa) in the presence of BSA FITC as a model drug. EVs were stained with anti-CD9 or anti-CD63 and the detection antibody was anti mouse IgG secondary antibody labelled with 6-nm gold particles (A,B). IgG1 isotype control was used as a control for anti-CD9 and anti-CD63 staining (C). Anti FITC secondary antibody labelled with 10-nm gold particles stained BSA FITC present on EVS (A–C). As controls, cell supernatant containing EVs with non-conjugated BSA processed and stained in the same manner (D–F).