Table 3.
Biochemical properties comparison among SNAP-tag, SsOGT and the relative H5 mutant.
| Features | SNAP-tag® | SsOGT | SsOGT-H5 | |
|---|---|---|---|---|
| Molecular weight (kDa) a | 23.0 | 17.0 | 17.0 | |
| Topt (°C) | 37.0 | 80.0 | 75.0 | |
| Relative activity | at 25.0°C | 80% | 25% | 50% b |
| at 37.0°C | 100% | 45% | 65% | |
| at 80°C | - | 100% | 95% | |
| Catalytic activity at 37 °C (M−1 s−1) | 2.8 × 104 | 2.8 × 103 | 1.6 × 104 | |
| pHopt | 6.0 | 7.5 | 6.0 | |
| Thermal stability T½ (°C) | 6 h (37) | 3 h (70) | 3 h (70) | |
| Thermal stability T½ at 37 °C (h) | 6 | >24 | >24 | |
| Additives | NaCl | <0.3 M | >1.0 M | >1.0 M |
| EDTA | no | yes | yes | |
| sarcosyl | no | >0.5% | >0.5% | |
| DDT | yes | no | no | |