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. 2020 Apr 19;21(8):2851. doi: 10.3390/ijms21082851

Figure 9.

Figure 9

Positive regulation of the MAPK and JAK/STAT signaling pathways by copaiba essential oil is independent of Akt3 expression. (A,B) cIEF electropherograms of ERK1/2 in (A) SH-SY5Y cells transfected with siRNA molecules that target Akt3 transcripts and (B) HepG2 cells that express Akt3 from plasmid DNA as a function of time after treatment with copaiba essential oil. (C) Relative concentrations of ERK1/2 phosphoisoforms as a function of time after treatment. (D,E) cIEF electropherograms of STAT in (D) SH-SY5Y cells transfected with Akt3 siRNA and (E) HepG2 cells transfected with Akt3-encoding plasmid DNA as a function of time after treatment with copaiba essential oil. (F) Relative concentrations of STAT3 as a function of time after treatment. The relative concentration describes the fold change in the expression level of a protein phosphoisoform after treatment compared to that under the control condition. The error bars indicate the standard deviations of six repeated measurements per experimental condition. The asterisks indicate a statistical significance of p ≤ 0.05 versus the control. SH-SY5Y and HepG2 cells were treated with 100 ng/mL copaiba essential oil.