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. 2020 Apr 13;21(8):2689. doi: 10.3390/ijms21082689

Figure 4.

Figure 4

Functional characterization of miRNAs in murine microglial cells (BV-2) and murine endothelial cells (C166). (A) Successful overexpression of miRNAs in both cell lines was verified via RT-qPCR 48 h after transfection. Overexpression was normalized to endogenous expression of the respective miRNA. Three independent experiments were performed, each with 2-3 technical replicates. Bars indicate standard errors (SEs). (B) Effect of miRNA overexpression on BV-2 cell viability, which was normalized to the viability of transfected cells with control miRNA cel-mir-39-3p. Seven independent experiments were performed, with 6-8 technical replicates each. Bars indicate SEs. (C) Effect of miRNA overexpression on the migration of BV-2 cells. A scratch assay was performed, and cell migration was defined as the percentage area closed after 16 h incubation. Seven independent experiments were performed, with 4-6 technical replicates each. Bars indicate SEs. * Q-value < 0.05 (Kruskal-Wallis test, followed by a Dunn’s multiple comparison test to receive uncorrected p-values for the comparisons of interest, which were corrected using FDR).