Table 1.
Kinetics of the spermatozoa during incubation under capacitating conditions before DGC, right after DGC (T0h) and every passing hour of incubation until T3 h. Abbreviations of CASA kinetics are detailed in the abbreviations section. Data are expressed as the mean ± SD.
| Motility | Before DGC | T0h | T1h | T2h | T3h |
|---|---|---|---|---|---|
| VCL (µm/s) | 76 ± 9 | 89 ± 11 | 88 ± 3 | 78 ± 10 | 71 ± 12 |
| VAP (µm/s) | 37 ± 4 | 41 ± 4 | 42.9 ± 1 | 40 ± 5 | 38 ± 8 |
| VSL (µm/s) | 27 ± 3 | 32 ± 3 | 37.7 ± 1 | 35 ± 4 | 34 ± 7 |
| LIN (%) | 37 ± 3 a | 38 ± 3 ab | 42 ± 1 | 46 ± 1 bc | 48 ± 2 c |
| STR (%) | 73 ± 7 a | 79 ± 3 ab | 85 ± 1 abc | 88 ± 1 bc | 89 ± 1 c |
| WOB (%) | 48 ± 3 | 46 ± 3 | 48 ± 2 | 51 ± 1 | 53 ± 3 |
| BCF (Hz) | 19 ± 0.7 a | 20.4 ± 0.4 ab | 22.4 ± 0.8 | 22.9 ± 0.8 bc | 24.1 ± 1 c |
| ALH (µm) | 2.9 ± 0.3 | 3.3 ± 0.3 | 3.38 ± 0.07 | 3 ± 0.07 | 2.8 ± 0.5 |
| Motility (%) | 56 ± 11 ab | 63 ± 9 a | 55 ± 11 ab | 46 ± 13 b | 43 ± 10 b |
Different letters indicate significant differences for each parameter among incubation times (p < 0.05, n = 6). DGC (density gradient centrifugation).