. 2020 Apr 15;21(8):2725. doi: 10.3390/ijms21082725

Table 2.

Percentages of acrosomal exocytosis, plasma membrane integrity and protein tyrosine phosphorylation (PTP) before and after (time 0 of incubation) sperm processing by density gradient centrifugation (DGC) and after 3 h incubation under capacitating conditions. At times 0 and 3 h, samples were treated for 30 min with 10 µM of calcium ionophore A23187. Data are expressed as the mean ± SD.

Sample	Incubation (h)	Positive Acrosomal Exocytosis (%)	Intact Plasma Membrane (%)	PTP-Positive Sperm
Sample	Incubation (h)	Positive Acrosomal Exocytosis (%)	Intact Plasma Membrane (%)	Head (%)	Equatorial Region (%)	Whole Flagella (%)
Before DGC		4.2 ± 4.5 ^a	58 ± 13 ^a	40 ± 15 ^b	31 ± 20 ^b	1.4 ± 1.4 ^a
Control	0	3.5 ± 1 ^a	59 ± 6 ^a	49 ± 18 ^ab	38 ± 23 ^b	1.4 ± 1.6 ^a
Ionophore	0	4.8 ± 2.5 ^ac	57 ± 10 ^a	50 ± 15 ^ab	43 ± 20 ^ab	0.8 ± 1 ^a
Control	3	9.3 ± 2.7 ^c	27 ± 16 ^c	61 ± 21 ^ac	54 ± 28 ^ac	9 ± 9.8 ^c
Ionophore	3	12.6 ± 4.6 ^c*	29 ± 13 ^c	59 ± 17 ^ac	52 ± 25 ^ac	11 ± 12 ^c

Different letters indicate significant differences at different incubation times under capacitating conditions (p < 0.05). An asterisk indicates significant differences between Control and ionophore (*p < 0.05, n = 6).