. 2020 Apr 15;21(8):2725. doi: 10.3390/ijms21082725

Table 3.

Distribution of protein tyrosine phosphorylation in bull spermatozoa showing intact acrosome (IA) or acrosomal exocytosis (AE). Data are expressed as mean ± SD.

Incubation Time (h)	Ionophore Treatment	Acrosomal Exocytosis	Analyzed Spermatozoa (n)	PTP Patterns
Incubation Time (h)	Ionophore Treatment	Acrosomal Exocytosis	Analyzed Spermatozoa (n)	I	II	III
Before DGC	no	IA	1356	64 ± 9 ^a	24 ± 12 ^b	12 ± 6 ^b
Before DGC	no	AE	60	78 ± 23 ^a	12 ± 20 ^b	12 ± 16 ^b
0	no	IA	1269	63 ± 7 ^a	29 ± 8 ^b	8 ± 2 ^c
0	no	AE	44	65 ± 26 ^a	30 ± 27 ^ab	5 ± 9 ^b
0	yes	IA	1410	56 ± 11 ^a****	36 ± 14 ^b***	8 ± 3 ^c**
0	yes	AE	71	90 ± 14 ^a	10 ± 14 ^b	0
3	no	IA	1222	54 ± 11 ^a*	36 ± 16 ^a**	10 ± 7 ^b*
3	no	AE	130	82 ± 9 ^a	15 ± 9 ^b	3 ± 6 ^b
3	yes	IA	1266	50 ± 13 ^a**	39 ± 21 ^b*	12 ± 8 ^c**
3	yes	AE	184	83 ± 8 ^a	11 ± 11 ^b	6 ± 5 ^b

Different letters indicate significant differences between the phosphorylation patterns within the same row (p < 0.05, n = 6). Asterisks indicate significant differences between reacted and no reacted of the same pattern (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, n = 6). DGC (density gradient centrifugation).