. 2020 Apr 15;21(8):2725. doi: 10.3390/ijms21082725

Table 4.

Distribution of protein tyrosine phosphorylation in bull spermatozoa showing intact (I) or compromised (C) plasma membrane. Data are expressed as mean ± SD.

Incubation Time (h)	Ionophore Treatment	Plasma Membrane Integrity	Analyzed Spermatozoa (n)	PTP Patterns
Incubation Time (h)	Ionophore Treatment	Plasma Membrane Integrity	Analyzed Spermatozoa (n)	I	II	III
Before DGC	no	I	711	59 ± 26 ^a	40 ± 22 ^a	0.1 ± 0.4 ^b*
Before DGC	no	C	510	51 ± 15 ^a	47 ± 19 ^a	1.4 ± 0.7 ^b
0	no	I	688	46 ± 26 ^a	43 ± 26 ^a*	10 ± 22 ^b
0	no	C	474	30 ± 17 ^b	63 ± 27 ^a	7 ± 10 ^c
0	yes	I	625	53 ± 20 ^a**	44 ± 32 ^a*	3 ± 3 ^b
0	yes	C	451	28 ± 12 ^b	68 ± 20 ^a	4 ± 7 ^b
3	no	I	318	35 ± 21 ^a*	62 ± 20 ^a*	2 ± 4 ^b
3	no	C	838	16 ± 6 ^b	83 ± 13 ^a	1 ± 1 ^c
3	yes	I	339	51 ± 21 ^a***	47 ± 21 ^a***	2 ± 2 ^b
3	yes	C	803	22 ± 16 ^b	77 ± 16 ^a	0.1 ± 0.4 ^c

Different letters indicate significant differences between the phosphorylation patterns within the same row (p < 0.05, n = 6). Asterisks indicate significant differences between intact and compromised sperm plasma membrane within the same PTP pattern (* p < 0.05, ** p < 0.01 and *** p < 0.001, n = 6). DGC (density gradient centrifugation).