Figure 3.
Monitoring bacterial and phage concentration during seed priming. (A) The bacterial titer (CFU/g seed) was followed as a function of time during a seven-day priming experiment in three independent repeats of non-treated and treated seeds in blue and orange, respectively. At day 0, the bacterial concentration was 108 CFU/g. The bacterial titer dropped 100-fold in the infected seeds after treatment with phages at a final concentration of 109 PFU/mL and remained stable over the course of the priming. (B) Phage titer (PFU/g seed) was followed over time in infected seeds. Twenty-four hours after treatment, the concentration of phage was 106 PFU/g and increased after 48 h to 108 PFU/g. After 72 h, the concentration dropped again to 107 PFU/g and remained quite stable until day 7 (106 PFU/g).