Figure 3.

JAK2/STAT1 and TAPs gene expression levels in PMF patients and healthy control subjects. The JAK2/STAT1 expression levels are closely linked to the PMF condition. During our analysis, we showed that JAK2/STAT1 gene expression levels were significantly upregulated in PB CD34⁺ cells of PMF patients compared to healthy controls subjects (a and b). Furthermore, the PMF patients who had the JAK2^V617F mutation had significantly upregulation of JAK2/STAT1 compared to wild-type. Transporters associated with Antigen Processing 1 and 2 are proteins that in humans are encoded by the TAP1 and TAP2 genes. These genes are involved in the degradation of peptides in order to assemble at the class I molecules. In CD34⁺ cells of PMF patients, we showed that TAP1 and TAP2 expression levels were significantly upregulated compared to healthy controls subjects (c). Furthermore, PMF JAK2^V617F mutated patients presented significantly upregulated levels of TAP1 and TAP2 compared to PMF JAK2^V617F wild-type. Data are expressed as z-score intensity expression levels and presented as vertical scatter dot plots. p values < 0.05 were considered to be statistically significant (* p < 0.05; ** p < 0.005; *** p < 0.0005; **** p < 0.00005).