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. 2020 Apr 23;21(8):2966. doi: 10.3390/ijms21082966

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Differential expression of tight junction molecules after injury in skin and oral keratinocytes. Immortalized human skin keratinocyte (HaCaT) and human gingival keratinocyte (TIGK) cells were cultured in a 12-well culture plate. When the cells were confluent, three horizontal and three vertical scratch wounds were made. mRNA expression of claudin (CLDN) CLDN1, CLDN4, occludin (OCLN), junctional adhesion molecule (JAM) JAM1, JAM2, JAM3, zonula occludens (ZO) ZO1, ZO2, and ZO3 were examined 0.5, 4, and 24 hours (h) after wounding by real-time PCR. UW: unwounded. n = 3 replicates, * p < 0.05, ** p < 0.01.