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Differential expression of tight junction molecules after lipopolysaccharide (LPS) treatment in skin and oral keratinocytes. HaCaT and TIGK cells were cultured in a 12-well culture plate. The cells were treated with LPS (100 ng/mL) when reaching 80% confluency. mRNA expression of CLDN1, CLDN4, OCLN, JAM1, JAM2, JAM3, ZO1, ZO2, and ZO3 was examined at 4 and 24 h after treatment by Real-Time PCR. Control: untreated. n = 3 replicates, * p < 0.05, ** p < 0.01.
