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Adipocytes autophagy was induced by Rapa treatment. (A) Cell viability were detected by CCK8 (n = 6). (B) Relative mRNA level of autophagy-related genes with or without Rapa for different lengths of time (n = 6). (C) Protein level of autophagy-related genes with or without Rapa for different lengths of time (n = 6). (D) MDC and DAPI staining by 100 nM Rapa treatment for 12 h. Values are means ± SD. vs. control group, * p < 0.05, ** p < 0.01.
