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Mark4 promoted Rapa-induced autophagy. (A) The overexpression or interference efficiency of Mark4 after the pretreatment of pcDNA-Mark4 or shRNA-Mark4 for 24 h (n = 6). (B) MDC staining and GFP-LC3 expression as indicated after transfection with Mark4 plasmid vector. Scale bar, 100 µm (n = 6). (C) Relative mRNA level of autophagy-related genes (D) Protein level of autophagy-related genes. Values are means ± SD. vs. control group, * p < 0.05, ** p < 0.01.
