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Mark4 promoted Rapa-induced autophagy via mTOR signaling pathway independent on AMPK/AKT signaling pathway. Adipocytes were pretreated with pcDNA-Mark4 or shRNA-Mark4 or Rapa or MK-2206 or Compound C. (A) Protein level of p-mTOR, t-mTOR, p-S6K, and S6K1 (n = 6). (B) Protein level of p-AKT and t-AKT (n = 6). (C) Protein level of p-AMPK and t-AMPK (n = 6). Values are means ± SD. vs. control group, * p < 0.05, N.S.
