Figure 9.
Evaluation of copper effects on VEGF165 (10 ng/mL), VEGF73-101, and VEGFI83G on HUVEC cells. (a) Cells (3 × 103 cells/wells) were treated for 24 h in EMB2 basal medium with 0.2% FBS without growth factors. (b) Cells (1.5 × 103 cells/wells) were treated for 24 h in EMB2 basal medium with 2% FBS with growth factors and without VEGF165. In the end, cell viability was evaluated by MTS assay. Values are expressed as the percentage of viable cells with respect to untreated cells (CTRL). Data are the mean ± SEM of three different experiments performed in triplicate. Statistically significant differences are indicated with * = p < 0.05 vs. CTRL (One-Way ANOVA + Tukey’s test).