Figure 7.

Relative mRNA levels of factors that could participate in viroid degradation in pollen: NtAGO5 (a,d), NtDCL (b,e) and NtTudor S-like nuclease (c,f). Result of qPCR performed as described in Materials and Methods were normalized to actin. Left: samples from AFCVd-infected or transformed plants; C and I, healthy control and infected sample, respectively; T, pLAT58:AFCVd-transformed/infected samples; L, young leaves; 3 and 5, pollen at developmental stages 3 and 5, respectively; G, germinating pollen (pollen tubes); M, mature pollen. Right: samples of 35S:CBCVd-transformed/infected plants; C and T, Lat52-transformed control and CBCVd-transformed/infected samples, respectively; L, young leaves; 3 and 5, pollen at developmental stage 3 and 5, respectively; M and G, mature CBCVd-transformed pollen and CBCVd-transformed pollen tubes, respectively.