Table 1.
Viroid | Inoculum | Inoculation Method | Number of Plants | |
---|---|---|---|---|
Inoculated | Infected | |||
AFCVd | Crude RNA from N. benthamiana | Carborundum | 12 | 1 |
AFCVd | Full length genomic restriction fragments SalI | biolistic | 12 | 0 |
AFCVd | RNA (native viroid ) from N. benthamiana | biolistic | 12 | 9 |
CBCVd | Crude RNA from N. benthamiana | Carborundum | 12 | 0 |
CBCVd | Full length genomic restriction fragments SacI | biolistic | 12 | 0 |
CBCVd | RNA (native viroid ) from N. benthamiana | biolistic | 12 | 0 |
Dot-blot analysis was performed two months post inoculation (Figure 2). Biolistic inoculation was performed: each plant was shot four times with either 100 ng of RNA or 50 ng of cDNA. Pre-purified 2 M-LiCl fraction of RNA for preparation of carrier.