Table 1.

AFCVd and CBCVd infection tests on Nicotiana tabacum.

Viroid	Inoculum	Inoculation Method	Number of Plants
			Inoculated	Infected ${}^{\mathit{a}}$
AFCVd	Crude RNA from N. benthamiana	Carborundum	12	1
AFCVd	Full length genomic restriction fragments SalI	biolistic ${}^b$	12	0
AFCVd	RNA (native viroid ${}^c$) from N. benthamiana	biolistic ${}^b$	12	9
CBCVd	Crude RNA from N. benthamiana	Carborundum	12	0
CBCVd	Full length genomic restriction fragments SacI	biolistic ${}^b$	12	0
CBCVd	RNA (native viroid ${}^c$) from N. benthamiana	biolistic ${}^b$	12	0

${}^a$ Dot-blot analysis was performed two months post inoculation (Figure 2). ${}^b$ Biolistic inoculation was performed: each plant was shot four times with either 100 ng of RNA or 50 ng of cDNA. ${}^c$ Pre-purified 2 M-LiCl fraction of RNA for preparation of carrier.