Figure 2.

Sweroside blocks the formation of ASC specks in primary macrophages. (A–C) Bone marrow-derived macrophages (BMDMs) were fixed, permeabilized, and stained for ASC (green). The nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI: blue). The arrows indicate ASC specks. The number of ASC specks per 100 × 100 µm² was obtained from different fields of view and is presented as a bar graph. The values represent the means ± SEM (n = 3). #, significantly different from vehicle alone, p < 0.05. *, significantly different from ATP, nigericin, or MSU alone, p < 0.05. ND, not detected. Scale bars = 20 µm.