Figure 3.

Sweroside alleviates hepatic inflammation in mice fed an MCD diet. C57BL/6 mice were fed a normal diet (NOR) or methionine-choline-deficient diet (MCD) for two weeks. Sweroside (5, 30 mg/kg) or MCC950 (20 mg/kg) was administered with daily intraperitoneal injections with the diets. (A) Serum AST and ALT levels were determined. (B) Hepatic mRNA levels for total macrophages (F4/80), inflammatory macrophages (Ly6c), and neutrophils (MPO). (C,D) Representative immunohistochemistry liver sections stained for F4/80 and MPO (200X and 400X). For A and B, the values represent means ± SEM (n = 8). #, significantly different from NOR + vehicle, p < 0.05. *, significantly different from MCD + vehicle, p < 0.05.