Figure 5.

Distribution of mICAM-1 in lacrimal glands (LGs) of BALB/c and NOD mice. Cryosections of LGs from 12-week-old male BALB/c and NOD mice were processed and imaged at low and high magnification using confocal microscopy. mICAM-1 was labeled with goat anti-mouse ICAM-1 primary antibody and AF-488 conjugated donkey anti-goat secondary antibody (green). F-actin was labeled with rhodamine phalloidin (red) and nuclei were labeled with 4′,6-diamidino-2-phenylindole (DAPI) (blue). (A) mICAM-1 expression is detected in lymphocytic infiltrates of the LG and correlated with the severity of the inflammation. (B) mICAM-1 expression was also observed in vascular endothelium, lymphocytes, and basolateral membranes of LG epithelial cells next to lymphocytes. White arrowheads (A): LG acini; white arrows (L): lymphocytes; * (V): vascular lumen.