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. 2020 May 5;11:805. doi: 10.3389/fmicb.2020.00805

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Copyright © 2020 Langlois, Millette, Guay, Dubé-Duquette, Chamberland, Jacques, Rodrigue, Bouarab, Marsault and Malouin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ROS production (RFI) in S. aureus Newbould and derivative mutants (A), and in S. aureus ATCC 29213 and derivative mutants (B). Strains were grown without antibiotic or in the presence of TO (4 × MIC for susceptible strains and 8 μg/ml for resistant strains), ciprofloxacin (CIP, at 4 × MIC), GEN (4 × MIC), or the combination of TO (8 μg/ml) and GEN (4 × MIC). The reducing agent cysteine (Cys, 20 mM) was added where specified. Statistical analyses were performed using a two-way ANOVA with a Dunnett’s multiple comparison test, comparing each group to their control without antibiotic; ****p < 0.0001 and **p < 0.01.