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. Author manuscript; available in PMC: 2020 May 12.
Published in final edited form as: J Vis Exp. 2020 Feb 21;(156):10.3791/60868. doi: 10.3791/60868

Figure 2. Representative double immunostaining on P21 FFPE mouse adrenals.

Figure 2.

The FFPE mouse adrenal sections were stained with two primary antibodies from rabbits in the following order: anti-β-catenin (in green, stains the outer cortex) and then anti-20αHSD (in red, stains the inner cortex). An 8-min stripping step was performed in between. Scale bar 100 μm. DAPI stains cell nuclei, in blue.