Table 2.
Comparison of multi-gene expression systems available for the BEVS
| MultiBac/OmniBac | LIC-based MacroBac | USER-based system | biGBac | GoldenBac | |
|---|---|---|---|---|---|
| Number of genes | up to 4 with Cre-lox, more possible via restriction/ligation | up to 8, more possible with additional cloning rounds |
up to 16 more possible with additional cloning rounds |
up to 25 | up to 15 |
| Expression of intermediates | no, only those in or fused to acceptors | yes | yes | yes | yes |
| Efficiency | Varies; triple assembly via Cre-lox has very low efficiency | 2 colonies to be screened at each step | 10 colonies to be tested for assembly of dual cassettes into multi-gene constructs | 6–12 colonies to be tested in first assembly step | 2 colonies tested for assembly of up to 12 genes |
| Construct verification | many possible variants with possibility of Tn7 element duplication | only 1 correct version at each step | only 1 correct version at each step | only 1 correct version at each step | only 1 correct version |
| Sequence requirement |
2–4, none 4+, no BstXI sites |
no PmeI, SwaI sites | none |
2–5, none 5+, no PmeI sites |
no BsaI sites |
| Number of assembly steps |
2–4 genes, 1 step 4+, 2 steps |
2 genes, 1 step, 4 genes, 2 steps, 8 genes, 3 steps |
2–4 genes, 1 step 4+, 2 steps and 2 bacmid integrations |
2–5 genes, 1 step 5+, 2 steps |
1 step |
| Method of Baculovirus generation | Tn7 transposase or homologous recombination | Tn7 transposase only |
Tn7 transposase only |
Tn7 transposase only | Tn7 transposase or homologous recombination |