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Fragment sizes of dsDNA, ssDNA and pure-ssDNA libraries. (a). Representative size distribution of different library preparation methods. ssDNA and pure-ssDNA methods showed significant enrichment of sub-100bp fragments. (b). ssDNA libraries showed 6–21 bp smaller fragment size than their matched dsDNA libraries. The size of pure-ssDNA libraries was 12–13 bp smaller in PS1 and PS2 than dsDNA libraries but similar to dsDNA libraries in PS6 to PS10
