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. 2020 May 12;20:161. doi: 10.1186/s12935-020-01217-8

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — HOXD-AS1 is upregulated in HCC. a The levels of HOXD-AS1 in HCC and normal tissues were measured using qPCR. ^**P<0.01 compared to normal. b The level of HOXD-AS1 was significantly higher in III~IV stage than that in I~II stage. ^*P<0.05 compared to I-II. c The levels of HOXD-AS1 in HCC tissues without and with distant metastasis. ^*P<0.05 compared to no metastasis. d The levels of HOXD-AS1 in HCC cells (HepG2, SMMC-7721, BEL7402 and HuH7) and normal hepatocytes cell line, LO2 were detected using qPCR assay. ^**P<0.01 compared to LO2 cell